fixable viability dye efluor 506 antibody (Thermo Fisher)

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Thermo Fisher fixable viability dye efluor 506 antibody
Fixable Viability Dye Efluor 506 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixable+viability+dye+efluor+506+antibody/live+dead+fixable+aqua+dead+cell+stain+kit/10__1016_slash_j__mtbio__2025__102075-60-15-9
Average 90 stars, based on 1 article reviews

fixable viability dye efluor 506 antibody - by Bioz Stars, 2026-09

90/100 stars

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Staining:

Article Title: PINK1 is a target of T cell responses in Parkinson’s disease
Article Snippet: .. Cells were then stained with a mixture of the following antibodies: Fixable Viability Dye eFluor 506 (Thermo Fisher), CD3-AF700 (BD, RRID:AB_10597906), CD4-BV711 (BD, RRID:AB_2740432), and CD8-BV650 (Biolegend, RRID:AB_11125174) for 30 minutes at 4°C in the dark. .. Stained cells were then washed twice and resuspended in 100 μL PBS to be run on an LSR II flow cytometer (BD; a detailed protocol can be found at https://doi.org/10.17504/protocols.io.bwu9pez6 ).

Article Title: The gut microbiome controls reactive astrocytosis during A β amyloidosis via propionate-mediated regulation of IL-17
Article Snippet: Cell debris was removed using Debris Removal Solution (130-109-398, Miltenyi Biotec), and a brain-derived single-cell suspension was made according to the manufacturer’s guidelines. .. Cell-surface staining was performed using the following antibodies: Fixable Viability Dye eFluor 506 (65-0866-14, Invitrogen, Thermo Fisher Scientific); CD45 BUV661 (1:400; 612975, clone: 30-F11, BD Biosciences); CD11b-APC-Cy7 (1:1,000; A15390, clone: M1/70; Invitrogen, Thermo Fisher Scientific); Ly6C BV421 (1:1,000; 562727, clone: AL-21, BD Biosciences); TCRgd BUV737 (1:200; 748991, clone: GL3, BD Biosciences);, CD3e BUV396 (1:100, 563565, clone: 145-2C11; BD Biosciences); CD8 PerCP-Cy5.5(1:67; 1299159, clone: 53-6.7, BD Biosciences); CD4 AF700 (1:333; 557956, clone: RM4-5, BD Biosciences); CD64 BV786 (1:83; 741024, clone: X54-5/7.1, BD Biosciences);, MHCII BV605 (1:200; 563413, clone: M5/114.15.2, BD Biosciences); and CD25 PE-Cy7 (1:200; 552880, clone: PC61, BD Biosciences). .. For intracellular staining, the Foxp3/Transcription Factor Staining Buffer Set (00-5523099; eBioscience) was used following the manufacturer’s guidelines.

Article Title: Dual-Responsive Epigenetic Inhibitor Nanoprodrug Combined with Oncolytic Virus Synergistically Boost Cancer Immunotherapy by Igniting Gasdermin E-Mediated Pyroptosis.
Article Snippet: Cancer immunotherapy has emerged as a promising approach for the treatment of various cancers.. However, the immunosuppressive tumor microenvironment (TME) limits the efficacy of current immunotherapies.. In this study, we designed a dual-responsive DNA methyltransferase inhibitor nanoprodrug ACNPs for combination therapy with oncolytic herpes simplex virus (oHSV).

Article Title: PINK1 is a target of T cell responses in Parkinson's disease
Article Snippet: .. Cells were then stained with a mixture of the following antibodies: Fixable Viability Dye eFluor 506 (Thermo Fisher), CD3-AF700 (BD, RRID:AB_10597906), CD4-BV711 (BD, RRID:AB_2740432), and CD8-BV650 (Biolegend, RRID:AB_11125174) for 30 min at 4°C in the dark. .. Stained cells were then washed twice and resuspended in 100 µL PBS to be run on an LSR II flow cytometer (BD; a detailed protocol can be found at (https://doi.org/10.17504/protocols.io.bwu9pez6).

Immunopeptidomics:

Article Title: Trigger inducible tertiary lymphoid structure formation using covalent organic frameworks for cancer immunotherapy
Article Snippet: .. 1) Fixable Viability Dye, 1:1000, eBioscience, eFluor 506, Cat 65-0866-14 2) anti-mouse CD45, 1:500, eBioscience, APC-Cyanine7, Clone I3/2.3, Cat A15395; 3) anti-mouse CD3e, 1:500, eBioscience, FITC, Clone 145-2C11, Cat 11-0031-82; 4) anti-mouse CD4, 1:500, eBioscience, eFluor 450, Clone RM4-5, Cat 48-0042-82; 5) anti-mouse CD8a, 1:500, Biolegend, PerCP, Clone 5H10, Cat MCD0831; 6) anti-mouse CD11b, 1:500, Biolegend, FITC, Clone M1/70, Cat 101206; 7) anti-mouse CD11c, 1:500, eBioscience, FITC, Clone N418, Cat 11-0114-82; 8) anti-mouse CD80, 1:300, eBioscience, PE, Clone 16-10A1, Cat 12-0801-82; 9) anti-mouse CD86, 1:300, eBioscience, APC, Clone GL1, Cat 17-0862-82; 10) anti-mouse MHC-II, 1:300, Biolegend, Bv421, Clone M5/114.15.2, Cat 25-5321-82; 11) anti-mouse MHC-II, 1:300, eF450, Clone AF6-120.1, Cat 48-5320-82; 12) anti-mouse CD44, 1:500, eBioscience, PE, Clone IM7, Cat 12-0441-82; 13) anti-mouse CD62L, 1:200, eBioscience, APC, Clone MEL-14, Cat 17-0621-82; 14) anti-mouse Ly6C, 1:200, Biolegend, APC, Clone HK1.4, Cat 128016; 15) anti-mouse Ly6G, 1:200, Biolegend, PE, Clone 1A8, Cat 127607 No customized antibodies were used. ..

Fluorescence:

Article Title: Fn -OMV potentiates ZBP1-mediated PANoptosis triggered by oncolytic HSV-1 to fuel antitumor immunity
Article Snippet: FlowJo 10 software (Version 10.0.6, Tree Star) was utilized to analyze and visualize the research results. .. For fluorescence labeling, the antibodies used included Fixable Viability Dye (1:1000; eFluor 506; eBioscience), anti-CD45 (1:500; APC-CY7; eBiosciences), anti-CD3 (1:500; FITC; eBiosciences), anti-CD4 (1:500; eFluor450; eBiosciences), anti-CD8 (1:500; PerCP-Cyanine 5.5; BioLegend), anti-CD11b (1:500; FITC; Biolegend), anti-CD11c (1:500; FITC; eBiosciences), anti-CD80 (1:300; PE; eBiosciences), anti-CD86 (1:300; APC; eBiosciences), anti-MHC-II (1:300; PE-Cyanine7; eBioscience), anti-CD25 (1:200; APC; eBioscience), anti-FOXP3 (1:100; PE; eBioscience), anti-CD44 (1:500; PE; eBiosciences), anti-CD62L (1:200; APC; eBiosciences), anti-F4/80 (1:200; PE-Cyanine7; eBioscience), anti-CD206 (1:200; APC; BioLegend), anti-CD86 (1:200; PE; BioLegend), and anti-PD-L1 (1:200; PE; Biolegend) following the manufacturers’ instructions. .. Apoptosis was detected using the Annexin V-FITC/PI Apoptosis Kit from MULTI SCIENCES.

Labeling:

Flow Cytometry:

Article Title: FMRP silencing via siRNA lipid nanoparticles to reprogram the tumor microenvironment and enhance anti-PD-1 efficacy in triple-negative breast cancer
Article Snippet: Ethanol and other chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). .. The antibodies used for flow cytometry were sourced from Thermo Fisher Scientific (USA) and included Fixable Viability Dye eFluor 506, anti-CD16/CD32, anti-CD45APC-eFluor 780, anti-CD3e-FITC, anti-IA/IE-PerCP-eFluor 710, antiCD8-PE-Cy7, anti-CD80-PE, and anti-CD86-PE-Cy7. .. TNF-α and IFN-γ ELISA kits were obtained from Biolegend (USA).

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